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BD5-2d

規(guī)格:

價(jià)格:

編號(hào):B206003

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

BD5-2d

商品貨號(hào)

B206003

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Tissue

spleen

培養(yǎng)基

Cell Type

hybridoma:lymphoblast B lymphocyte; somatic cell hybri

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications

Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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Derivation

Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein. Spleen cells were fused with SP2/O myeloma cells (ATCC CRL 1581). The antibody recognizes substantially purified p100, human neu related protein, the full-length HER-2/neu molecule in tumor tissue (p185) or the extracellular domain (ECD) (p105) in serum, plasma, cell cultures and fluids.

Comments

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing

Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to reach. 1 X 10(6) cells/ml.

Interval: Maintain cultures at a cell concentration between 6 x 10(4) and 6 X 10(5) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Isotype

mouse IgG1 kappa

Name of Depositor

Applied Biotechnology, Inc

U.S. Patent Number

5,401,638

References

Carney WP, McKenzie SJ. Detection and quantification of neu related proteins in the biological fluids of humans. US Patent 5,401,638 dated Mar 28 1995

Carney WP, et al. Detection of neu p185 in cell lysates. US Patent 5,604,107 dated Feb 18 1997

McKenzie SJ, et al. Generation and characterization of monoclonal antibodies specific for the human neu oncogene product, p185. Oncogene 4: 543-548, 1989. PubMed: 2566965

Animals were immunized with NIH/3T3 cells expressing full-length human HER-2/neu protein.

Weinberg RA, et al. Oncogenes and methods for their detection. U.S. Patent 6,713,619 dated Mar 30 2004

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