| 產品名稱 | BD5-2d |
|---|---|
| 商品貨號 | B206003 |
| Organism | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue | spleen |
| Cell Type | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications | Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein. |
| Storage Conditions | liquid nitrogen vapor phase |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images |  |
| Derivation | Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein. Spleen cells were fused with SP2/O myeloma cells (ATCC CRL 1581). The antibody recognizes substantially purified p100, human neu related protein, the full-length HER-2/neu molecule in tumor tissue (p185) or the extracellular domain (ECD) (p105) in serum, plasma, cell cultures and fluids. |
| Comments | Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein. Spleen cells were fused with SP2/O myeloma cells (ATCC CRL 1581). The antibody recognizes substantially purified p100, human neu related protein, the full-length HER-2/neu molecule in tumor tissue (p185) or the extracellular domain (ECD) (p105) in serum, plasma, cell cultures and fluids. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing | Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to reach. 1 X 10(6) cells/ml. Interval: Maintain cultures at a cell concentration between 6 x 10(4) and 6 X 10(5) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype | mouse IgG1 kappa |
| Name of Depositor | Applied Biotechnology, Inc |
| U.S. Patent Number | |
| References | Carney WP, McKenzie SJ. Detection and quantification of neu related proteins in the biological fluids of humans. US Patent 5,401,638 dated Mar 28 1995 Carney WP, et al. Detection of neu p185 in cell lysates. US Patent 5,604,107 dated Feb 18 1997 McKenzie SJ, et al. Generation and characterization of monoclonal antibodies specific for the human neu oncogene product, p185. Oncogene 4: 543-548, 1989. PubMed: 2566965 Animals were immunized with NIH/3T3 cells expressing full-length human HER-2/neu protein. Weinberg RA, et al. Oncogenes and methods for their detection. U.S. Patent 6,713,619 dated Mar 30 2004 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
