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pET-His

規格:

價格:

編號:B206134

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pET-His

商品貨號

B206134

Designations

pET-His

Depositors

T Hai

培養基

Biosafety Level

Host

E. coli HB101

Vector Information

Size (kb): 4.636

Vector: pET-His (plasmid)

Promoters: Promoter T7 (phi10)

Construction: pET3a

Marker(s):ampR

Construct size (kb): 4.636

Features: marker(s): ampR
promoter: T7 (phi10)
replicon: pMB1
MCS: NdeI...BamHI
terminator: phi10

Applications

expression vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; BamHI--4.6; BglI--2.5, 2.1, 0.25.

The plasmid contains the following restriction sites (kb from nt 1): BamHI--4.12; BglI--0.87, 3.19, 3.42; BglII--3.99; EcoRI--4.63; HindIII--4.60; NcoI--4.12; NdeI--4.09; PstI--0.75; PvuI--0.62; PvuII--2.29; SalI--3.71; XbaI--4.05; XhoI--4.11.

Digestion with NcoI, BamHI or XhoI and subsequent filling of ends, allows cloning of blunt-ended fragments with a +0, +1 (C) or +2 (GA) nucleotide reading frame, respectively.

Fusion proteins can be purified using a nickel-chelating column, even under denaturing conditions.

Constructed from pET3a by insertion of a double stranded oligonucleotide linker between the NdeI and BamHI sites, creating three restriction sites and encoding six His residues.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Chen BP, Hai T. Expression vectors for affinity purification and radiolabeling of proteins using Escherichia coli as host. Gene 139: 73-75, 1994. PubMed: 8112591

Shipped

freeze-dried

Shipping Information

?Freeze dried E. coli containing the plasmid

B194256：pET-HisK

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