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phi4-2 [CMCC 3125, Ch35:Taq#4-2]
phi4-2 [CMCC 3125, Ch35:Taq#4-2]
規(guī)格:
貨期:
編號:B206239
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 phi4-2 [CMCC 3125, Ch35:Taq#4-2]
商品貨號 B206239
Designations phi4-2 [CMCC 3125, Ch35:Taq#4-2]
GenBank Number

J04639

Species Thermus aquaticus Brock and Freeze
Depositors Cetus Corp., A Belt, Cetus Corp.
Applications
produces protein DNA polymerase I
Vector
Construct size (kb): 45.0
Insert
DNA: genomic
Insert lengths(kb): 15.0
Gene product: Taq DNA polymerase [polI]
Target Gene: DNA polymerase I
Insert Size (kb) 15.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.0, 15.0, 10.7; BamHI--19.3, 10.7, 4.55, 3.85, 2.6 (approximate); HindIII--19.3, 10.7, 8.0, 4.5, 0.8, 0.58.
A HindIII digest gives 4 fragments from the insert (kb): 8.0 kb, 4.5 kb, 0.8 kb, and 0.58 kb.
The 8.0 kb fragment is sufficent to express a thermostable DNA polymerase activity and a 60 kDa polypeptide immunologically related to Taq DNA polymerase in Escherichia coli.
The 4.5 kb fragment is also available in pFC83, ATCC 67422.
References

Lawyer FC, et al. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264: 6427-6437, 1989. PubMed: 2649500

Gelfand DH, et al. Purified thermostable enzyme. US Patent 4,889,818 dated Dec 26 1989

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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