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pSW253
pSW253
規格:
價格:
編號:B206341
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSW253
商品貨號
B206341
Designations
pSW253
GenBank Definition

cloning vector pBR322, complete genome

培養基
GenBank Number

K02537

J01749

Species
reticuloendotheliosis virus-A
Depositors
TJ Jacoby
Vector
Construct size (kb): 19.70
Insert
DNA: genomic
Insert lengths(kb): 15.3
Insert Size (kb)
15.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Frozen glycerol stock of E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--15.3, 4.3; HindIII--7.3, 6.0, 4.1, 1.3, 1.0; BamHI--9.7, 6.0, 2.2, 1.8.
The insert contains the following restriction sites (approximate kb from one end): HindIII--0.3 (spleen DNA), 7.8, 9.3 (viral); KpnI--2.7, 3.6, 5.6 (viral), 14.5 (spleen); SalI--3.0 (viral); XhoI--6.7 (viral); SacI--2.0, 2.8, 9.9 (viral), 11.3, 13.2.
Size of construct: 19.7kb.
Full-length clone of reticuloendotheliosis virus-A (pSW253) inserted at EcoRI site in pBR322; construct is amp resistant and tet resistant. See also GenBank/EMBL/DDBJ J01749.
Corresponds to the larger EcoRI fragment of clone 2-20-6. GenBank K02357 begins 170 bp upstream of the XhoI site.
The insert contains the 8.3 kb REV-A proviral DNA and flanking chicken spleen sequences subcloned from clone 2-20-6. The proviral sequences begin about 2 kb from the EcoRI site closer to the HindIII site.
References

Chen IS, et al. Characterization of reticuloendotheliosis virus strain T DNA and isolation of a novel variant of reticuloendotheliosis virus strain T by molecular cloning. J. Virol. 40: 800-811, 1981. PubMed: 6275117

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