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pAV2
pAV2
規格:
價格:
編號:B206397
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAV2
商品貨號
B206397
Designations
pAV2
Depositors
CA Laughlin
培養基
Biosafety Level
1
Vector Information
Size (kb): 8.7
Vector: pAV2 (plasmid)
Promoters: Promoter P19
Construction: pA11P.Xba, AAV genome
Marker(s):ampR
Construct size (kb): 8.7
Features: marker(s): ampR
promoter: viral P5, P19, P40
replicon: pMB1, AAV
terminator: AAV polyadenylation
Applications
contains sequence human adeno-associated virus type 2
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BglII--4.7, 3.7; EcoRI--6.6, 1.8.
pAV1 (ATCC 37215) and pAV2 (ATCC 37216) both contain the complete AAV2 genome, but in opposite orientations. The pAV2 vector has fewer polylinker sites than that of pAV1 (HindIII, ClaI, and one EcoRI site have been deleted).
Contains the complete viral genome for use in site-specific mutagenesis studies. Transfection into human 293 cells with an Ad helper virus results in infectious AAV virus particles.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Laughlin CA, et al. Cloning of infectious adeno-associated virus genomes in bacterial plasmids. Gene 23: 65-73, 1983. PubMed: 6352411

Wondisford FE, et al. Cloning of the human thyrotropin beta-subunit gene and transient expression of biologically active human thyrotropin after gene transfection. Mol. Endocrinol. 2: 32-39, 1988. PubMed: 3398841

Shipped
frozen
Shipping Information
Distributed: frozen
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