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pJ5Eomega
pJ5Eomega
規格:
價格:
編號:B206467
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pJ5Eomega
商品貨號
B206467
Designations
pJ5Eomega
Depositors
H Land
培養基
Biosafety Level
1
Vector Information
Size (kb): 3.7000000476837160
Vector: pJ5Eomega (plasmid)
Promoters: Promoter MMTV LTR
Construction: pBR322, SV40, MMTV, MSV
Marker(s):ampR
Construct size (kb): 3.700000047683716
Features: marker(s): ampR
promoter: MMTV LTR
replicon: pMB1
terminator: SV40
enhancer: MSV
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; HindIII--3.7; BamHI--3.7; SmaI--3.7.
Demonstrates expression inducible by dexamethasone.
A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the MMTV LTR promoter and MSV enhancer.
Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation A site), and MMTV LTR (-360 to +150, glucocorticoid regulatory sequences, cap site) and MSV enhancer.
Media
ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C
References

Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225

Hartmut Land, personal communication

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
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