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YCpIF7

規(guī)格:

價格:

編號:B206488

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產品名稱

YCpIF7

商品貨號

B206488

Designations

YCpIF7

Depositors

RW Davis, PK Foreman

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 6.4000000953674320

Vector: YCpIF7 (plasmid)

Promoters: Promoter for expression GAL1

Construction: pUN95

Marker(s):ampR,HIS3

Construct size (kb): 6.400000095367432

Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SalI...SacI
centromere: CEN4

Applications

YX-type (expression) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): XbaI--6.6; BamHI--4.2, 2.4; EcoRI--6.6.

One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.

Vectors YCpIF7, YCpIF8 and YCpIF9 (ATCC 87084-87086) differ only in the reading frame of the multiple cloning site.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907