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pHIL6R.32
pHIL6R.32
規格:
價格:
編號:B206778
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHIL6R.32
商品貨號
B206778
Designations
pHIL6R.32
GenBank Number

M20566

培養基
Species
Homo sapiens, human
Depositors
GH Fey
Applications
The sequence was confirmed by partial sequence analysis and it includes and matches nucleotides 440 to 1649 of the published sequence.
Vector
Construct size (kb): 4.169000148773193
Insert
DNA: cDNA
Insert lengths(kb): 1.205000042915344
Tissue: HL-60 cell line (promyelocytic), differentiated
Gene product: interleukin 6 receptor [IL6R]
Insert Size (kb)
1.205
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert includes the following restriction sites (in nucleotides from the 5' end of the published sequence): SmaI--580, PstI--638, SacI--1201.
Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.9, 1.2; HindIII--3.9, 0.9; BamHI--4.1; PstI--3.15, 1.0; SalI--4.1; EcoRI/XbaI--2.9, 1.3; BamHI/XhoI--2.9, 1.3.
The sequence was confirmed by partial sequence analysis and it includes and matches nucleotides 440 to 1649 of the published sequence. It encodes most of the protein coding region (437 - 1841).
The insert is oriented such that linearizing with BamHI and using the T3 promoter gives the antisense strand, and linearizing with EcoRI and using the T7 promoter gives the sense strand.
References

Baumann M, et al. Molecular cloning, characterization and functional expression of the rat liver interleukin 6 receptor. J. Biol. Chem. 265: 19853-19862, 1990. PubMed: 2174054

Yamasaki K, et al. Cloning and expression of the human interleukin-6 (BSF-2/IFN-beta2) receptor. Science 241: 825-828, 1988. PubMed: 3136546

Georg H Fey, personal communication

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