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pSE3.2-L
pSE3.2-L
規(guī)格:
價格:
編號:B206807
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pSE3.2-L
商品貨號
B206807
Designations
pSE3.2-L
Species
Homo sapiens, human
培養(yǎng)基
Depositors
HF Willard
Vector
Construct size (kb): 6.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 3.200000047683716
Gene product: DNA Segment, single copy [DXS16]
Alleles: B1, B1, B2, B2, A1, A2
Insert Size (kb)
3.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Digest with HindIII will release two insert fragments of 1.5 and 1.7 kb. The vector is 3.0 kb.
Restriction digests of the clone give the following sizes (kb): HindIII--2.9, 1.8, 1.7; PvuI--uncut; BamHI--3.7, 2.4; SalI--3.3, 2.9; EcoRI/HindIII--2.9, 1.8, 1.7; consistent with deposited DNA.
This probe is 2 cM (LOD score = 14.96) from pD2 (DXS43), 3 cM (LOD score = 8.56) from 782 (DXS85) and 15 cM (LOD score = 6.05) from dic56 (DXS143).
Codominant, X-linked segregation demonstrated in several large, 3-generation families.
Low background with final wash in 0.1X SSC at 65C.
Obtained from a recombinant lambda phage identified by a chromosome walk from pXUT23 (ATCC 57154/5). This is 22 kb from pXUT23.
Enzyme(s) not detecting polymorphism: BamHI, BglII, HindiII, TaqI in a screen of 4 female Caucasians.
References

Brown CJ, Willard HF. MspI RFLP detected with chromosome-walk clone pXUT23-SE3.2L from DXS16 in Xp22.1-22.3. Nucleic Acids Res. 15: 9614, 1987. PubMed: 2446265

Huntington F Willard, personal communication

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