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pAL7

規格:

價格:

編號:B207104

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pAL7

商品貨號

B207104

Designations

pAL7

Depositors

H Okayama

培養基

Biosafety Level

Vector Information

Size (kb): 6.5000000000000000

Vector: pAL7 (plasmid)

Construction: pFL20, pDB262

Marker(s):LEU2,ampR

Construct size (kb): 6.5

Features: marker(s): ampR, LEU2
replicon: pMB1, ars

Applications

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.3, 1.2, 0.9; HindIII--6.6; PstI--6.6.

Plasmid linearized with PstI can be used to introduce cDNA libraries constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe, resulting in the formation, by homologous recombination, of concatamers maintained as episomal DNA.

Shares pBR322 sequences from the PvuII site to the EcoRI site with pcD.

Contains the ars and stb sequences from Schizosaccharomyces pombe and the LEU2 gene from Saccharomyces cerevisiae. The PstI site interrupts the beta-lactamase gene.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Okazaki K, et al. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 18: 6485-6489, 1990. PubMed: 2251111

Shipped

freeze-dried

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