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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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mKV1.3-EC
mKV1.3-EC
規格:
價格:
編號:B207180
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
mKV1.3-EC
商品貨號
B207180
Designations
mKV1.3-EC
GenBank Number

M30441

培養基
Species
Mus musculus, mouse
Depositors
G Chandy
Applications
construct to generate mRNA that can be expressed in Xenopus oocytes potassium voltage-gated channel, shaker-related subfamily, member 3 potassium voltage-gated channel, shaker related subfamily, type n
Vector
Construct size (kb): 5.0
Insert
DNA: genomic
Insert lengths(kb): 1.76800000667572
Gene product: potassium voltage-gated channel, shaker-related subfamily, member 3(potassium voltage-gated channel, shaker related subfamily, type n) [Kcna3]
Target Gene: potassium voltage-gated channel, shaker-related subfamily, member 3
Insert Size (kb)
1.768
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglII/BstEII--3.2, 1.8; BstEII--5.0; BglII--5.0; BamHI--5.0; EcoRI--5.0.
The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.00; BanI--0.02; NcoI--0.44; PstI--1.02; SacII--0.15.
For in vitro transcription, linearize with EcoRI and use SP6 RNA polymerase.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Grissmer S, et al. Expression and chromosomal localization of a lymphocyte K+ channel gene. Proc. Natl. Acad. Sci. USA 87: 9411-9415, 1990. PubMed: 2251283

Krieg PA, Melton DA. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Res. 12: 7057-7070, 1984. PubMed: 6207484

George Chandy, personal communication

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