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Der p1 - clone 3e
Der p1 - clone 3e
規(guī)格:
價(jià)格:
編號(hào):B207191
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Der p1 - clone 3e
商品貨號(hào)
B207191
Designations
Der p1 - clone 3e
GenBank Number

S66377

培養(yǎng)基
Species
Dermatophagoides pteronyssinus, house dust mite (HDM)
Depositors
NA Kalsheker
Applications
contains sequence cysteine protease
Vector
Construct size (kb): 3.349999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: BamHI
Vector end: XbaI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: house dust mite (HDM)
Gene symbol: Der p1
Gene name: cysteine protease
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert end: Modification: BamHI linker
Insert end: Modification: XbaI linker
Insert size (kb): 0.7
Cross references: DNA Seq. Acc.: S66377
Nucleotides 21-686 of the insert correspond to
nucleotides ~344-1009 of S66377.
Insert lengths(kb): 0.699999988079071
Gene product: cysteine protease [Der p1]
Target Gene: cysteine protease
Insert Size (kb)
0.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--2.7, 0.7; HindIII--3.0, 0.35; PvuII--2.3, 0.75, 0.3.
Insert contains the following restriction sites (approximate kb from the 5' end): ClaI--0.06, 0.30, 0.64; HindIII--0.41; NdeI--0.67; PstI--0.03; PvuII--0.05.
Insert contains the complete coding sequence for the mature peptide plus the following 20 bp sequence at the 5' end: 5' - CTAATGTGATTTCCAAGCGC - 3'.
References

Scobie G, et al. Expression cloning of a dust mite cysteine proteinase, Der p1, a major allergen associated with asthma and hypersensitivity reactions. Biochem. Soc. Trans. 22: 448S, 1994. PubMed: 7698465

Chua KY, et al. Sequence polymorphisms of cDNA clones encoding the mite allergen Der p I. Int. Arch. Allergy Immunol. 101: 364-368, 1993. PubMed: 8353459

Noor A Kalsheker, personal communication

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