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pGEM-hIRF
pGEM-hIRF
規(guī)格:
價(jià)格:
編號:B207206
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pGEM-hIRF
商品貨號
B207206
Designations
pGEM-hIRF
GenBank Number

Z11559

培養(yǎng)基
Species
Homo sapiens, human
Depositors
LC Kuhn
Vector
Construct size (kb): 6.498000144958496
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-7Zf(-)
Intact vector size: 3.000
Type of vector: phagemid
Vector end: EcoRI
Vector end: KpnI
Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI
HindIII BamHI SacI BstXI NsiI
Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI
HindIII BamHI SacI BstXI NsiI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IREB1
Other gene symbols: IRE1; FRP*; IRF*
Gene name: iron-responsive element binding protein 1
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9
Strain: fibroblast cell line EK4
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 3.498
Cross references: DNA Seq. Acc.: Z11559
Insert lengths(kb): 3.497999906539917
Tissue: placenta
Gene product: aconitase(iron-responsive element binding protein 1) [FRP*]
Insert Size (kb)
3.498
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XbaI/ClaI--3.55, 3.0; EcoRI--3.1, 1.35, 1.3, 0.7; PstI--6.55; BamHI--3.4, 3.15; HindIII--6.2, 0.5.
The insert contains the following restriction sites (bp from the 5' end): BamHI--143; BglI--712, 2262, 2493, 2854; EcoRI--1368, 2015, 2220; HindIII--3040; KpnI--2384; PstI--364; PvuII--2154, 3104.
A probe derived from sequence between nt 44 and 344 detects a single genomic EcoRI fragment of approximately 4.3 kb in human placental DNA.
Contains 107 bp of 5' untranslated region, the complete coding sequence and 720 bp of 3' untranslated region.
Detects a single mRNA of approximately 4.0 kb in the fibroblast cell line EK4.
Constructed from clones isolated from placenta and fibroblast libraries.
References

Hirling H, et al. Expression of active iron regulatory factor from a full-length human cDNA by in vitro transcription/translation. Nucleic Acids Res. 20: 33-39, 1992. PubMed: 1738601

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