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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 9.3000001907348630

DESCRIPTION OF VECTOR:
Intact vector size: 9.300
Type of vector: plasmid
Cloning sites: NotI ClaI BamHI
Polylinker sites: NotI EcoRI ClaI HindIII XbaI BglII PstI BamHI NotI
Construction: pHSS6, 2 micron
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): kanR, TRP1
replicon: pMB1, 2 micron

Vector: pIA2 (plasmid)

Construction: pHSS6, 2 micron

Marker(s):TRP1,kanR

Construct size (kb): 9.300000190734863

Features: marker(s): kanR, TRP1
replicon: pMB1, 2 micron

YE-type (episomal) shuttle vector

shuttle vector

vector for other uses

Restriction digests of the clone give the following sizes (kb): HindIII--4.8, 2.2, 1.4, 1.2; BamHI--9.6; NotI--9.6; ClaI--9.6.

In S. cerevisiae, requires a host expressing FLP such as YPH485 or YPH494, or a host modified by pFV17 (ATCC 77170).

When propagated in a strain carrying an integrated copy of GAL10-FLP, the copy number is 10-20 without induction and 200-400 when induced by galactose.

YE-type shuttle vector permitting FLP-driven high copy number.

Constructed by inserting a BalI/EcoRV fragment of the 2 micron circle (interrupting FLP) into the SmaI site of pHSS6 which had been modified by the addition of a TRP1 gene.

The order of the major features in this plasmid is: REP3 - D - REP1 - REP2 - TRP1 - MCS (NotI - BamHI/NotI) - kanR.

Temperature: 37.0°C