| 產(chǎn)品名稱 |
pSC101-trp.I15 |
| 商品貨號(hào) |
B207258 |
| Designations |
pSC101-trp.I15 |
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
Sanraku-Ocean Co., Ltd. |
| Applications |
Host is a mutant derived from Escherichia coli W3110 (ATCC 27325) and is trpAE1 trpR tnaA. |
| Vector |
Construct size (kb): 24.60000038146973 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSC101
Intact vector size: 9.263
Type of vector: plasmid
Cloning sites: EcoRI
Polylinker sites:
Construction: natural isolate
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pSC101
Cross references: DNA Seq. Acc.: X01654 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSC101
Intact vector size: 9.263
Type of vector: plasmid
Cloning sites: EcoRI
Polylinker sites:
Construction: natural isolate
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pSC101
Cross references: DNA Seq. Acc.: X01654 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: trp
Gene name: tryptophan operon
Contains complete coding sequence?: U
Localization: 28 min
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 15.6
Cross references: DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: trp
Gene name: tryptophan operon
Contains complete coding sequence?: U
Localization: 28 min
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 15.6
Cross references: Insert lengths(kb): 15.60000038146973 Gene product: tryptophan operon [trp] |
| Insert Size (kb) |
15.600 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Host is a mutant derived from Escherichia coli W3110 (ATCC 27325) and is trpAE1 trpR tnaA. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Imanaka T, et al. Phenotypic stability of trp operon recombinant plasmids in Escherichia coli. J. Gen. Microbiol. 118: 253-261, 1980. PubMed: 6999123
Aiba S, et al. New approach to tryptophan production by Escherichia coli: genetic manipulation of composite plasmids in vitro. Appl. Environ. Microbiol. 43: 289-297, 1982. PubMed: 7036897
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
