黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pJIR750 [JIR2438] B207389
pJIR750 [JIR2438]
pJIR750 [JIR2438]
規(guī)格:
價格:
編號:B207389
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pJIR750 [JIR2438]
商品貨號
B207389
Designations
pJIR750 [JIR2438]
Depositors
JI Rood
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 6.5679998397827150
Vector: pJIR750 (plasmid)
Promoters: Promoter lac
Construction: pJIR418
Marker(s):cmlR
Construct size (kb): 6.567999839782715
Features: insert detection: lacZ'
marker(s): catP
other: rep
promoter: lac
replicon: pIP404
replicon: pMB1
MCS: EcoRI...HindIII
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BglI--4.4, 2.2; ClaI--6.6; EcoRV--6.6.
The vector contains the following restriction sites (approximate kb from the EcoRI site): BglI--0.20, 2.50; ClaI--4.99, 5.03; EcoRV--4.72; NdeI--0.27, 2.09; PvuI--0.17; PvuII--0.14, 6.39; SmaI--4.98.
The chloramphenicol resistance gene (catP), expressed in both C. perfringens and E. coli, was derived from C. perfringens and is inactivated by insertions at the EcoRV site.
The standard reverse sequencing primer is not useful in this vector due to the duplication of the 181 bp region between pUC18 sites PvuII and EcoRI.
Shuttle expression vector derived from pJIR418 (ATCC 77387) permitting expression of antibiotic resistance in both hosts.
Media
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Bannnam TL, Rood JI. Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. Plasmid 29: 233-235, 1993. PubMed: 8356117

Shipped
frozen
Shipping Information

?Frozen glycerol stock of E. coli containing the plasmid.

  • 王經(jīng)理
    手機(jī)號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號：13626845108
    QQ：972239479