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pCllase 1 [pX7]
pCllase 1 [pX7]
規格:
價格:
編號:B207495
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCllase 1 [pX7]
商品貨號
B207495
Designations
pCllase 1 [pX7]
GenBank Number

X05231

培養基
Species
Homo sapiens, human
Depositors
HJ Rahmsdorf
Vector
Construct size (kb): 5.099999904632568
Insert
DNA: cDNA
Insert lengths(kb): 2.053999900817871
Tissue: fibroblast, PMA-treated
Gene product: matrix metalloproteinase 1 (interstitial collagenase)(collagenase, epidermolysis bullosa, dystrophic, (autosomal recessive), collagenase, fibroblast, collagenase, interstitial) [CLG]
Insert Size (kb)
2.054
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 0.3; BamHI--3.8, 1.3; PstI--5.1; HindIII--5.1; AvaI--5.1; HindIII/AccI--3.1, 2.0; HindIII/BglII--5.1; HindIII/SmaI--3.1, 2.0.
Contains the complete coding sequence. Transcription from the SP6 promoter yields RNA complementary to the mRNA. If the first nucleotide after the dC tail is counted as 1, the poly(A) tract of 75 bp begins at nt 1969.
The insert contains XbaI, EcoRI, XmnI, EcoRV, SacI, BamHI, PstI, XbaI, BglII, and HindII sites.
The first ATG (nt 67-69) opens a reading frame of 469 aa. The 5' untranslated region contains a CCATC ribosome binding site (nt 26-30).
The sequence TTATTTAT (nt 1597-1604) in the 3' untranslated region is also found in mRNA for TNF, IL1, fibronectin, CSF, and interferons.
Enzyme(s) not detecting polymorphism: TaqI, MspI, BglII, RsaI, BamHI, XmnI, SacI, PvuII, EcoRV.
References

Whitham SE, et al. Comparison of human stromelysin and collagenase by cloning and sequence analysis. Biochem. J. 240: 913-916, 1986. PubMed: 3030290

Angel P, et al. 12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5'-flanking region. Mol. Cell. Biol. 7: 2256-2266, 1987. PubMed: 3037355

Spurr NK, et al. Restriction fragment length polymorphism analysis and assignment of the metalloproteinases stromelysin and collagenase to the long arm of chromosome 11. Genomics 2: 119-127, 1988. PubMed: 2900807

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