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Rhizamoeba sp.
Rhizamoeba sp.
規(guī)格:
價(jià)格:
編號(hào):B207573
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Rhizamoeba sp.
商品貨號(hào)
B207573
Strain Designations
BSJ-04190019
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

培養(yǎng)基
Isolation
sediment, saltwater
Beaufort North Carolina, United States
Product Format
frozen
Storage Conditions
liquid nitrogen vapor phase
Type Strain
no
Medium
ATCC® Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 20.0°C
Duration: xenic; Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831) and as a food source
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Subcultivation
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Name of Depositor
JC Cole, TA Nerad
Special Collection
NSF - Protistology
References

sediment from a man-made marsh island at the edge of a dredge spoilage site

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