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42
42
規格:
價格:
編號:B207604
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
42
商品貨號
B207604
Designations
42
GenBank Number

M87857

培養基
Species
Homo sapiens, human
Depositors
MA Gitt
Vector
Construct size (kb): 40.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJB8
Intact vector size: 5.400
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI EcoRI
Polylinker sites:
Construction: HomerI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LGALS2
Other gene symbols: GALBP
Genomic copy number: unique
Gene name: lectin, galactoside-binding, soluble, 2 (galectin 2)
Contains complete coding sequence?: U
Chromosome: 22; Localization: 22 q12-q13
Tissue: leukocyte
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 35.0
Cross references: DNA Seq. Acc.: M87857
DNA Seq. Acc.: M87858
DNA Seq. Acc.: M87859
DNA Seq. Acc.: M87860
Insert lengths(kb): 35.0
Tissue: leukocyte
Gene product: lectin, galactoside-binding, soluble, 2 (galectin 2) [GALBP]
Insert Size (kb)
35.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; BamHI/EcoRI--9.5, 7.5, 5.8, 3.0, 2.5, 1.85, 1.8, 1.6, 1.5, 1.05, 1.0, 0.75, 0.55, 0.55;
HindIII/EcoRI--12.5, 4.55, 3.6, 3.0, 2.9, 2.7, 2.5, 1.75, 1.7, 1.2, 1.08, 1.08, 1.0, 0.7, 0.55; KpnI/EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; SalI/EcoRI--15.0, 7.5, 5.0, 2.7, 2.5, 2.5, 1.85, 1.8, 1.05, 1.0, 0.55.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Contains the complete gene (4 exons).
References

Lau YF, Kan YW. Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin cluster. Proc. Natl. Acad. Sci. USA 80: 5225-5229, 1983. PubMed: 6310566

Gitt MA, et al. Isolation and expression of a gene encoding L-14-II, a new human soluble lactose-binding lectin. J. Biol. Chem. 267: 10601-10606, 1992. PubMed: 1375225

Gitt MA, Barondes SH. Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30: 82-89, 1991. PubMed: 1988031

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