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Bovine adenovirus 1
Bovine adenovirus 1
規格:
價格:
編號:B207646
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Bovine adenovirus 1
商品貨號
B207646
Classification
Adenoviridae, Mastadenovirus, Bovine mastadenovirus A
Deposited As
Bovine adenovirus type 1
培養基
Agent
Bovine adenovirus 1
Strain
10
Applications
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Feces of normal calf
Product Format
frozen 1.0 mL
Storage Conditions
-70°C or colder
Images
ATCC VR-313 images
Comments
REFERENCE STRAIN: Pereira HG et al. A short description of the Adenovirus group. Virology 20: 613-620, 1963.

Agglutinates rat RBC. CPE may be minimal, frequently demonstrating less than 25% involvement of cell sheet, even with prolonged incubation.  AAV reported to be present.
Effect on Host
CPE, cell rounding with vacuolization, clumping, and sloughing
Recommended Host
MDBK (NBL-1) cells (ATCC® CCL-22™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
M Klein
Source
Feces of normal calf
References

Klein M, et al. Isolation from cattle of a virus related to human adenovirus. Proc. Soc. Exp. Biol. Med. 102: 1-4, 1959. PubMed: 14409689

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

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