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hp35
hp35
規格:
價格:
編號:B207726
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
hp35
商品貨號
B207726
Designations
hp35
GenBank Number

M38443

M65271

培養基
Species
Homo sapiens, human
Depositors
U Gubler
Applications
The cDNA contains two in-frame Met codons: one at position 100-102 and the second one at position 202-204. Only the second Met codon was used for expression of bioactive IL-12 in mammalian cells by the depositor.
Vector
Construct size (kb): 4.064000129699707
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRV
Vector end: EcoRV
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IL12A
Other gene symbols: IL-12 p35
Gene name: interleukin 12A (natural killer cell stimulatory factor 1,
cytotoxic lymphocyte maturation factor 1, p35
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 p25-p26.2
Type of DNA: cDNA
Insert 5' end: EcoRV
Insert 3' end: EcoRV
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: M38443
Nucleotides 33 - 1058 of the insert correspond to
nucleotides 1 - 1026 of M38443.
DNA Seq. Acc.: M65271
Nucleotides 33 - 1058 of the insert correspond to
nucleotides 1 - 1026 of M65271.
Insert lengths(kb): 1.100000023841858
Gene product: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35 [IL12A]
Insert Size (kb)
1.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): NotI/SalI--2.9, 1.2; HindIII--3.4, 0.65; EcoRV--4.1.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.42; HindIII--0.63; BglII--0.66.
The cDNA contains two in-frame Met codons: one at position 100-102 and the second one at position 202-204. Only the second Met codon was used for expression of bioactive IL-12 in mammalian cells by the depositor.
The insert, p35 cDNA including 5' and 3' noncoding regions,was PCR amplified out of a lambda phage clone and ligated into the EcoRV site of pBluescript SK+.
References

Gubler U, et al. Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc. Natl. Acad. Sci. USA 88: 4143-4147, 1991. PubMed: 1674604

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