Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
This endometrium cell line was derived from the stromal cells obtained from an adult female with myomas.
The primary stromal endometrium cells were immortalized by infection with supernatant from the packaging cell line pA317-hTERT (Geron Corp.; Menlo Park, CA) which expressed the hTERT and the puromycin resistance genes.
Volumes are given for T-75 flasks; proportionally reduce or increase the amount of dissociation medium for culture vessels of other sizes.
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Krikum G, et al. A novel immortalized human endometrial stromal cell line with normal progestational response. Endocrinology 145: 2291-2296, 2004. PubMed: 14726435
Krikun G, et al. Endometrial angiopoietin expression and modulation by thrombin and steroid hormones: a mechanism for abnormal angiogenesis following long-term progestin-only contraception.. Am. J. Pathol. 164: 2101-2107, 2004. PubMed: 15161644
Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13.
Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988
Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
