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HHCPB34
HHCPB34
規(guī)格:
價格:
編號:B207765
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HHCPB34
商品貨號
B207765
Designations
HHCPB34
GenBank Number

M78893

培養(yǎng)基
Species
Homo sapiens, human
Applications
The predicted product size is (bp): 66. The observed product size(s) is(are) (bp): 69 (human).
Vector
Construct size (kb): 3.700000047683716
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D1S330E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens cyclic AMP-regulated phosphoprotein, 19 kDa
Chromosome: 1; Localization: 1
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M78893; EST: EST01041; Primer: M13 Forward
DNA Seq. Acc.: M78894; EST: EST01042; Primer: M13 Reverse;
THC id: THC91006
Insert lengths(kb): 0.800000011920929
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: cyclic AMP-regulated phosphoprotein, 19 kDa [ARPP19*]
Insert Size (kb)
0.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.8; BamHI--3.7; HindIII--3.6, 0.2; PstI--3.5, 0.25; PvuII--2.5, 1.2.
EST01041 can be detected in genomic DNA using the following oligonucleotides (5'-3'): AGAAGCCCTTTCCTGTTG and GTTCAAGGTCTTGGTGCTCA.
EST01041 matches PIR accession B35308 with 86.35% identity and 95.45% similarity over the sequence range: 3-68.
This sequence record is for bovine cyclic AMP-regulated phosphoprotein, 19 kDa.
The predicted product size is (bp): 66. The observed product size(s) is(are) (bp): 69 (human).
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Durkin AS, et al. Chromosomal assignment of human brain expressed sequence tags (ESTS) by analzying fluorescently-labeled PCR products from hybrid cell panels. Cytogenet. Cell Genet. 65: 86-91, 1994. PubMed: 8404072

Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994

J C Venter, personal communication

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