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SQMK-FP
SQMK-FP
規(guī)格:
價(jià)格:
編號:B207920
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SQMK-FP
商品貨號
B207920
Organism
Saimiri boliviensis boliviensis, monkey, bolivian squirrel
Tissue
kidney; tubule
培養(yǎng)基
Cell Type
epithelial
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
1 day newborn
Gender
male
Applications
The cell line can be used to study the biology of the squirrel monkey in vitro.
The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey.
SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
hyperdiploid with chromosome numbers ranging from 52 to 57 (submetacentric chromosome pair 15 and acrocentric chromosome pair 16). RefScammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921
Images
Derivation
SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey. Normal kidney tissue was minced and cultured. Proliferating cells were subsequently cloned by the limiting dilution. SQMK-FP cells have cell junctions with radiating filaments, indicating desmosomes and numerous surface projections containing longitudinally oriented filaments typical of tubular epithelium. The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey. The cell line can be used to study the biology of the squirrel monkey in vitro.
Clinical Data
male
Comments
SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey. Normal kidney tissue was minced and cultured. Proliferating cells were subsequently cloned by the limiting dilution. SQMK-FP cells have cell junctions with radiating filaments, indicating desmosomes and numerous surface projections containing longitudinally oriented filaments typical of tubular epithelium. The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey. The cell line can be used to study the biology of the squirrel monkey in vitro.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    An inoculum of 5 X 10(4) to 1 X 10(5) viable cells/cm2 is recommended. Do not exceed 3 X 10(6) cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 2 X 10(6) cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every two to three days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time
approximately 22 hours
Name of Depositor
JG Scammell
Deposited As
Saimiri boliviensis boliviensis
Year of Origin
1997
References

Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921

Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921

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