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pLGdelta312-polylinker

規格:

價格:

編號:B207969

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pLGdelta312-polylinker

商品貨號

B207969

Designations

pLGdelta312-polylinker

Depositors

DJ Stillman

培養基

Biosafety Level

Vector Information

Size (kb): 10.1999998092651400

Vector: pLGdelta312-polylinker (plasmid)

Promoters: Promoter CYC1 promoter (including CYC1 UAS)

Construction: pBR322

Marker(s):URA3,ampR

Construct size (kb): 10.19999980926514

Features: marker(s): URA3
marker(s): ampR
promoter: CYC1 promoter (including CYC1 UAS)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ

Applications

YE-type (episomal) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): XhoI--10.6; HindIII--8.0, 2.25; BglII--10.6.

This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511, 87512 & 87522). They differ by polylinker sites.

DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity.

Growth Conditions

Temperature: 37.0°C