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pPYRDH
pPYRDH
規(guī)格:
價格:
編號:B208056
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pPYRDH
商品貨號
B208056
Designations
pPYRDH
GenBank Number

M94065

培養(yǎng)基
Species
Homo sapiens, human
Applications
in another host, produces protein dihydroorotate dehydrogenase
Vector
Construct size (kb): 6.929999828338623
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pFL61
Intact vector size: 5.425
Type of vector: plasmid
Vector end: BstXI
Vector end: BstXI
Cloning sites: NotI
Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI
Construction: pUC19
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
replicon: pMB1, 2 micron
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DHODH
Gene name: dihydroorotate dehydrogenase
Contains complete coding sequence?: Y
Localization: 16 q22
Tissue: HeLa D98-H2 cell line
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: M94065
Insert lengths(kb): 1.5
Tissue: HeLa D98-H2 cell line
Gene product: dihydroorotate dehydrogenase [DHODH]
Target Gene: dihydroorotate dehydrogenase
Insert Size (kb)
1.5
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): NotI--5.5, 1.5; BamHI--4.6, 2.3; BglI--3.6; 1.9, 1.1, 0.4; PvuII--4.8, 2.1; ClaI--4.2, 2.35, 0.5.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.42; BglI--0.87, 1.25; ClaI--0.83; PstI--0.14, 0.29, 0.71; PvuII--1.06; SphI--1.3.
Contains the complete coding sequence (nt 1-1191).
Can complement ura1 mutants in Saccharomyces cerevisiae.
Detects a unique 1.6 kb transcript in humans.
References

Minet M, et al. Cloning and sequencing of a human cDNA coding for dihydroorotate dehydrogenase by complementation of the corresponding yeast mutant. Gene 121: 393-396, 1992. PubMed: 1446837

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