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pBSE36

規格:

價格:

編號:B208184

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pBSE36

商品貨號

B208184

Designations

pBSE36

GenBank Number

M34077

培養基

Species

tobacco mild green mosaic tobamovirus (TMGMV)

Depositors

F Garcia-Arenal

Vector

Construct size (kb): 5.099999904632568

Insert

DNA: cDNA

Insert lengths(kb): 1.871999979019165

Gene product: genome, partial

Insert Size (kb)

1.872

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 2.0; PstI--3.4, 1.7; PvuII--2.8, 2.3.

Insert corresponds to nt 1990 to nt 3862 of the genome. This includes coding sequences for the 183 kDa protein (exons nt 71 to nt 3403 and nt 3407 to nt 4900) and the 126 kDa protein (nt 71 to nt 3406).

Linearizing with HindIII and transcription from the T7 promoter gives the minus (antisense) strand. Linearizing with EcoRI and transcription from the T3 promoter gives the plus (sense) strand.

Classification

Tobamovirus

References

Garcia-Arenal F. Sequence and structure at the genome 3' end of the U2-strain of tobacco mosaic virus, a histidine-accepting tobamovirus. Virology 167: 201-206, 1988. PubMed: 3188396

Dracopoli NC, et al. The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology 177: 553-558, 1990. PubMed: 2371769

F Garcia-Arenal, personal communication

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