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pRLalpha1I3/23J

規格:

價格:

編號:B208358

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pRLalpha1I3/23J

商品貨號

B208358

Designations

pRLalpha1I3/23J

GenBank Number

J03552

培養基

Species

Rattus norvegicus, rat

Depositors

GH Fey

Vector

Construct size (kb): 7.599999904632568

Insert

DNA: cDNA

Insert lengths(kb): 4.599999904632568

Tissue: liver, male, acute phase

Gene product: alpha-1-inhibitor III, group 1 [A1I3A]

Insert Size (kb)

4.600

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The vector does not have convenient restriction sites close to the insert. A BamHI digest yields 3 fragments from the insert, 5' 0.7, 0.575, 3.5 kb 3'. The end fragments contain vector sequences, and the vector band is about 3 kb.

The insert contains the following restriction sites (nucleotide position according to GenBank J03552): BamHI--583, 1158; EcoRI--746, 3814.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.6, 3.0; HindIII--3.0, 2.8, 1.75, 0.4; XbaI--uncut; PstI--3.0, 2.0, 1.3, 0.9, 0.4; BamHI--3.4, 2.9, 0.7, 0.6.

The insert contains the complete coding sequence, which extends from nucleotide 80 to 4513. The signal peptide is encoded from 80 to 151.

References

Braciak TA, et al. Sequence and acute phase regulation of rat alpha1-inhibitor III messenger RNA. J. Biol. Chem. 263: 3999-4012, 1988. PubMed: 2831216

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