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購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Microvascular Endothelial Cell Growth Kit-VEGF
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  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Microvascular Endothelial Cell Growth Kit-VEGF
Microvascular Endothelial Cell Growth Kit-VEGF
規(guī)格:
貨期:
編號(hào):B208382
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 


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      產(chǎn)品名稱
      Microvascular Endothelial Cell Growth Kit-VEGF
    
    
    
    
      商品貨號(hào)
      B208382
    
    
      
    
      Biosafety Level
      1 


    

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
    		
    
    
      
    
      Product Format
      frozen 1 kit
    
    
      
    
      Storage Conditions
      -20°C (or -70°C for long-term storage)
    
    
      
    
      Comments
      
    The Microvascular Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for microvascular endothelial cells isolated from normal human tissue. The final concentration for each component in complete endothelial growth medium is as follows:
    

      
    
    • rh VEGF: 5 ng/ml
      • 
    
    • rh EGF: 5 ng/ml
      • 
    
    • rh FGF basic: 5 ng/ml
      • 
    
    • rh IGF-1: 15 ng/ml
      • 
    
    • L-glutamine: 10 mM
      • 
    
    • Heparin sulfate: 0.75 Units/ml
      • 
    
    • Hydrocortisone: 1 µg/ml
      • 
    
    • Ascorbic acid: 50 µg/ml
      • 
    
    • Fetal bovine serum: 5% 
      • 

    

    		
    
    
      
    
      Complete Growth Medium
      
      
    
    1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
      2. 
    
    2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
      3. 
    
    3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
      4. 
    
    4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
      5. 
    
    5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
      6. 
    
      
    
      Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC® PCS-110-040), add the indicated volume for each component:  
      
    
      
    
      
        
            
      
                 
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
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      Volume
      
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      Volume
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
       
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      		
    
      
      
      
      Sterility Tests
      
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      Final Concentration
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
       
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      		
    
      
      
      
      Functional Tests
      
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      Bovine Brain Extract (BBE)
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
       
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      		
    
      
      
      
      C of A
      
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      1.0 mL
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
       
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      
                
      		
    
      
          

      

						
					
					
      
							
      
							  
							  
							    
      
							      梅經(jīng)理
							      17280875617
							      1438578920
							    
      
							    
      
							      胡經(jīng)理
							      13345964880
							      2438244627
							    
      
							    
      
							      周經(jīng)理
							      17757487661
							      1296385441
							    
      
							    
      
							      于經(jīng)理
							      18067160830
							      2088210172
							    
      
							    
      
							      沈經(jīng)理
							      19548299266
							      2662369050
							    
      
							    
      
							      李經(jīng)理
							      13626845108
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