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pSK-hGCS1
pSK-hGCS1
規格:
價格:
編號:B208594
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSK-hGCS1
商品貨號
B208594
Designations
pSK-hGCS1
GenBank Number

M90656

培養基
Species
Homo sapiens, human
Depositors
R Mulcahy
Applications
The 764 bp PstI fragment (derived from the open reading frame) has been used as a probe for the human and rat sequences.
Vector
Construct size (kb): 4.639999866485596
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GCS*
Gene name: gamma-glutamylcysteine synthetase
Contains complete coding sequence?: U
Tissue: kidney
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.679
Cross references: DNA Seq. Acc.: M90656
Insert lengths(kb): 1.679000020027161
Tissue: kidney
Gene product: gamma-glutamylcysteine synthetase( glutamylcysteine synthetase, gamma, gamma-glutamylcysteine synthetase, light subunit) [GCS*]
Insert Size (kb)
1.679
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.65; BglII--4.65; HindIII--4.65; PvuII--2.45, 1.45, 0.75; PstI--3.7, 0.8, 0.15.
The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.463; NcoI--0.65; PstI--0.14, 0.9; PvuII--0.44; SacI--0.9; XbaI--1.44.
The first 120 bp of the insert do not match the corresponding sequences of the full-length cDNA and presumably represent cloning artifacts.
Detects human transcripts of 4.0 and 3.2 kb.
The 764 bp PstI fragment (derived from the open reading frame) has been used as a probe for the human and rat sequences.
Encodes 387 aa (of 637 aa) at the carboxyl end of the protein, and includes 398 nt of 3' untranslated sequence. The portion of this clone derived from the cDNA therefore corresponds approximately to nt 845 - 2400 of the sequence record.
References

Gipp JJ, et al. Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase. Biochem. Biophys. Res. Commun. 185: 29-35, 1992. PubMed: 1350904

Bailey HH, et al. Increase in gamma-glutamylcysteine synthetase activity and steady-state messenger RNA levels in melphalan-resistant DU-145 human prostate carcinoma cells expressing elevated glutathione levels. Cancer Res. 52: 5115-5118, 1992. PubMed: 1355406

R. Timothy Mulcahy, personal communication

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