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pMev

規格:

價格:

編號:B208686

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pMev

商品貨號

B208686

Designations

pMev

GenBank Number

M97382

培養基

Species

Cricetulus griseus, hamster, Chinese

Depositors

JL Goldstein

Vector

Construct size (kb): 9.300000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Rc/CMV7S
Intact vector size: 6.000
Type of vector: plasmid
Vector end: SalI
Vector end: NotI
Cloning sites:
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR
promoter: CMV
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: hamster, Chinese
Gene symbol: MEVT*
Gene name: mevalonate transporter
Contains complete coding sequence?: U
Tissue: MET-18b-2 cell line
Type of DNA: cDNA
Insert 5' end: SalI
Insert 3' end: NotI
Insert size (kb): 3.345
Cross references: DNA Seq. Acc.: M97382

Insert lengths(kb): 3.34500002861023

Tissue: MET-18b-2 cell line

Gene product: mevalonate transporter(monocarboxylate transporter 1) [MEVT*]

Insert Size (kb)

3.345

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): NotI/SalI--6.0, 3.3; SalI--9.3; NotI--9.3; EcoRI--6.1, 2.2; XhoI--8.0, 1.0, 0.3.

The insert contains the following restriction sites (approximate kb from the 5 ' end): SmaI--0.05, 2.9; EcoRI--2.1; XhoI--2.3.

Transcription of the insert is regulated by the CMV promoter, and transcription of neoR is regulated by the SV40 early promoter.

The ends of the insert were derived from linker adapters.

References

Otto JC, Casey PJ. The hepatitis delta virus large antigen is farnesylated both in vitro and in animal cells. J. Biol. Chem. 271: 4569-4572, 1996. PubMed: 8617711

Kim CM, et al. cDNA cloning of MEV, a mutant protein that facilitates cellular uptake of mevalonate, and identification of the point mutation responsible for its gain of function. J. Biol. Chem. 267: 23113-23121, 1992. PubMed: 1429658

Joseph L Goldstein, personal communication

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