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6G4.2.5

規格:

價格:

編號:B208787

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

6G4.2.5

商品貨號

B208787

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Tissue

spleen

培養基

Cell Type

hybridoma:lymphoblast B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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Derivation

An expression plasmid p6G425chim2 (ATCC 97055) was created to code for a chimeric murine-human Fab fragment of the monoclonal antibody. Cell lines producing the recombinant humanized antibody are available (ATCC CRL-12444 and ATCC CRL-12445)

Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against rabbit interleukin 8 (IL-8)

Cellular Products

immunoglobulin; monoclonal antibody; against rabbit interleukin 8 (IL-8)

Comments

Animals were immunized with rabbit interleukin 8 (IL-8).

Spleen cells were fused with P3X63Ag8U.1 mouse myeloma cells.

The antibody blocks IL-8 binding to rabbit neutrophil receptors.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10 exp6 cells/ml.

Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Evey 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Isotype

IgG2a kappa , IgG2a

Name of Depositor

Genentech, Inc.

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

5,677,426

References

Fong S, et al. Anti-IL-8 antibody fragments. US Patent 5,677,426 dated Oct 14 1997

Hsei V, et al. Methods of treating inflammatory diseases with anti-IL-8 antibody fragment-polymer conjugates. US Patent 6,468,532 dated Oct 22 2002

Hsei V, et al. Methods of treating inflammatory disease with anti-IL-8 antibody fragment-polymer conjugates. US Patent 6,458,355 dated Oct 1 2002

Gonzalez TN, et al. Humanized anti-IL-8 monoclonal antibodies. US Patent 6,133,426 dated Oct 17 2000

Gonzalez TN, et al. Humanized anti-IL-8 monoclonal antibodies. US Patent 6,117,980 dated Sep 12 2000

Gonzalez TN, et al. Nucleic acids encoding humanized anti-IL-8 monoclonal antibodies. US Patent 6,025,158 dated Feb 15 2000

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