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p35-1
p35-1
規(guī)格:
價(jià)格:
編號(hào):B208804
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p35-1
商品貨號(hào)
B208804
Designations
p35-1
GenBank Number

M13509

培養(yǎng)基
Species
Homo sapiens, human
Depositors
W Stetler-Stevenson
Vector
Construct size (kb): 4.96999979019165
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcD-X
Intact vector size: 2.800
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Features (with orientation and position when available):
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MMP1
Other gene symbols: CLG
Gene name: matrix metalloproteinase 1 (interstitial collagenase)
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 q21-q22
Strain: A2058 melanoma cell line
Tissue: brain metastasis
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 2.2
Cross references: DNA Seq. Acc.: M13509
Insert lengths(kb): 2.200000047683716
Tissue: brain metastasis
Gene product: matrix metalloproteinase 1 (interstitial collagenase)(collagenase, epidermolysis bullosa, dystrophic, (autosomal recessive), collagenase, fibroblast, collagenase, interstitial) [CLG]
Insert Size (kb)
2.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.45, 0.75; EcoRI--5.3; XhoI--3.0, 2.3; SacI--5.3; PstI--2.5, 1.5, 1.3.
The insert contains the following restriction sites (bp from the 5' end of the cDNA sequence): BamHI--1290; EcoRI--274; HincII--1951; SacI--1010; PstI--1468; XbaI--138, 1570.
The insert sequence is essentially identical to that reported for fibroblast collagenase except for noted changes.
Contains the complete coding sequence (nt 68-1478 of the cDNA). The 2.2 kb XhoI fragment includes some sequence from the vector: 175 bp at the 5' end and 50 bp at the 3' end.
References

Templeton NS, et al. Cloning and characterization of human tumor cell interstitial collagenase. Cancer Res. 50: 5431-5437, 1990. PubMed: 2167156

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