Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 70-80% confluent. Remove medium, wash cell monolayer once with PBS, and inoculate with a small volume (eg. 1 mL per cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 5-9 days at 37ºC in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Schmidt NJ, et al. An apparently new enterovirus isolated from patients with disease of the central nervous system. J. Infect. Dis. 129: 304-309, 1974. PubMed: 4361245
