| 產(chǎn)品名稱(chēng) |
pFEN207 |
| 商品貨號(hào) |
B208890 |
| Designations |
pFEN207 |
| GenBank Number |
M64618
|
| Species |
Chlamydia trachomatis |
| Depositors |
Rocky Mountain Laboratory, FE Nano, Rocky Mountain Laboratory |
| Applications |
produces protein 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) |
| Vector |
Construct size (kb): 9.2 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Chlamydia trachomatis
Gene symbol: gseA
Gene name: 3-deoxy-D-manno-octulosonic acid transferase (Kdo
transferase)
Contains complete coding sequence?: Y
Strain: LGV-434 (serovar L2)
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 6.5
Cross references: DNA Seq. Acc.: M64618 Insert lengths(kb): 6.5 Gene product: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) [gseA] Target Gene: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) |
| Insert Size (kb) |
6.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 2.8; EcoRI/HindIII--2.7, 1.35 (doublet), 0.84 (triplet), 0.64, 0.36, 0.27; ScaI/SacII--4.6, 2.4, 1.4, 0.96. Expresses the genus-specific lipopolysaccharide epitope of Chlamydia. The insert contains at least 2 open reading frames. The 1.4 kb ScaI/SacII fragment contains the complete coding sequence of gseA and has been sequenced. |
| Classification |
Chlamydiaceae, Chlamydia |
| References |
Nano FE, Caldwell HD. Recombinant clones of Chlamydia trachomatis lipopolysaccharide. US Patent 5,075,228 dated Dec 24 1991
Nano FE, Caldwell HD. Expression of the Chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli. Science 228: 742-744, 1985. PubMed: 2581315
Belunis CJ, et al. A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specfic epitope. J. Biol. Chem. 267: 18702-18707, 1992. PubMed: 1382060
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
