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pFEN207

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編號:B208890

品牌:Mingzhoubio

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RNA

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產品名稱

pFEN207

商品貨號

B208890

Designations

pFEN207

GenBank Number

M64618

培養基

Species

Chlamydia trachomatis

Depositors

Rocky Mountain Laboratory, FE Nano, Rocky Mountain Laboratory

Applications

produces protein 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase)

Vector

Construct size (kb): 9.2

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:

Insert

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Chlamydia trachomatis
Gene symbol: gseA
Gene name: 3-deoxy-D-manno-octulosonic acid transferase (Kdo
transferase)
Contains complete coding sequence?: Y
Strain: LGV-434 (serovar L2)
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 6.5
Cross references: DNA Seq. Acc.: M64618

Insert lengths(kb): 6.5

Gene product: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase) [gseA]

Target Gene: 3-deoxy-D-manno-octulosonic acid transferase (Kdo transferase)

Insert Size (kb)

6.5

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 2.8; EcoRI/HindIII--2.7, 1.35 (doublet), 0.84 (triplet), 0.64, 0.36, 0.27; ScaI/SacII--4.6, 2.4, 1.4, 0.96.

Expresses the genus-specific lipopolysaccharide epitope of Chlamydia.

The insert contains at least 2 open reading frames. The 1.4 kb ScaI/SacII fragment contains the complete coding sequence of gseA and has been sequenced.

Classification

Chlamydiaceae, Chlamydia

References

Nano FE, Caldwell HD. Recombinant clones of Chlamydia trachomatis lipopolysaccharide. US Patent 5,075,228 dated Dec 24 1991

Nano FE, Caldwell HD. Expression of the Chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli. Science 228: 742-744, 1985. PubMed: 2581315

Belunis CJ, et al. A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specfic epitope. J. Biol. Chem. 267: 18702-18707, 1992. PubMed: 1382060

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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