Restriction digests of the clone give the following sizes (kb): KpnI--6.2; BamHI--6.2.
A cloning vector that uses the plasmid primer approach of Okayama & Berg to allow expression of cDNA clones in yeast.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Miyajima A, et al. Analysis of full-length cDNA clones carrying GAL1 of Saccharomyces cerevisiae: a model system for cDNA expression. Nucleic Acids Res. 12: 6397-6414, 1984. PubMed: 6206472
