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pB3HE1
pB3HE1
規格:
價格:
編號:B209115
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pB3HE1
商品貨號
B209115
Designations
pB3HE1
Species
Brome mosaic virus, Brome mosaic virus
培養基
Depositors
P Ahlquist
Applications
Contains a noncoding sequence to serve as a probe for brome mosaic virus.
The insert corresponds to the 3' 200 bases of BMV RNA3. Because this region is 97-99% conserved among the BMV RNAs, the SP6 transcript of HindIII-linearized pB3HE1 hybridizes to all four BMV RNAs.
Vector
Construct size (kb): 3.099999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.2000000029802322
Gene product: noncoding sequence
Insert Size (kb)
0.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.2; EcoRI--2.9, 0.2. EcoRI/HindIII digest on acrylamide gel gives the same sizes as above.
Contains a noncoding sequence to serve as a probe for brome mosaic virus.
The insert corresponds to the 3' 200 bases of BMV RNA3. Because this region is 97-99% conserved among the BMV RNAs, the SP6 transcript of HindIII-linearized pB3HE1 hybridizes to all four BMV RNAs.
Derived from the 3' 200 bp HindIII/EcoRI fragment of pB3PM1.
Classification
Bromoviridae, Bromovirus
References

French R, Ahlquist P. Intercistronic as well as terminal sequences are required for efficient amplification of brome mosaic virus RNA3. J. Virol. 61: 1457-1465, 1987. PubMed: 3573144

Ahlquist P, Janda M. cDNA cloning and in vitro transcription of the complete brome mosaic virus genome. Mol. Cell. Biol. 4: 2876-2882, 1984. PubMed: 6549346

Ahlquist P, et al. Near identity of 3' RNA secondary structure in bromoviruses and cucumber mosaic virus. Cell 23: 183-189, 1981. PubMed: 7214524

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