| 產品名稱 |
pB3HE1 |
| 商品貨號 |
B209115 |
| Designations |
pB3HE1 |
| Species |
Brome mosaic virus, Brome mosaic virus |
| Depositors |
P Ahlquist |
| Applications |
Contains a noncoding sequence to serve as a probe for brome mosaic virus. The insert corresponds to the 3' 200 bases of BMV RNA3. Because this region is 97-99% conserved among the BMV RNAs, the SP6 transcript of HindIII-linearized pB3HE1 hybridizes to all four BMV RNAs. |
| Vector |
Construct size (kb): 3.099999904632568 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.2000000029802322 Gene product: noncoding sequence |
| Insert Size (kb) |
0.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Shipped: ?Freeze dried E. coli containing the plasmid. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.2; EcoRI--2.9, 0.2. EcoRI/HindIII digest on acrylamide gel gives the same sizes as above. Contains a noncoding sequence to serve as a probe for brome mosaic virus. The insert corresponds to the 3' 200 bases of BMV RNA3. Because this region is 97-99% conserved among the BMV RNAs, the SP6 transcript of HindIII-linearized pB3HE1 hybridizes to all four BMV RNAs. Derived from the 3' 200 bp HindIII/EcoRI fragment of pB3PM1. |
| Classification |
Bromoviridae, Bromovirus |
| References |
French R, Ahlquist P. Intercistronic as well as terminal sequences are required for efficient amplification of brome mosaic virus RNA3. J. Virol. 61: 1457-1465, 1987. PubMed: 3573144
Ahlquist P, Janda M. cDNA cloning and in vitro transcription of the complete brome mosaic virus genome. Mol. Cell. Biol. 4: 2876-2882, 1984. PubMed: 6549346
Ahlquist P, et al. Near identity of 3' RNA secondary structure in bromoviruses and cucumber mosaic virus. Cell 23: 183-189, 1981. PubMed: 7214524
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