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p101JdeltaE5.0 [p101Jdelta1E5.0, p101delta1 5.0E]
p101JdeltaE5.0 [p101Jdelta1E5.0, p101delta1 5.0E]
規(guī)格:
價(jià)格:
編號(hào):B209217
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p101JdeltaE5.0 [p101Jdelta1E5.0, p101delta1 5.0E]
商品貨號(hào)
B209217
Designations
p101JdeltaE5.0 [p101Jdelta1E5.0, p101delta1 5.0E]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Temple University, CM Croce, Temple University
Applications
Has been used to detect t(10;14)(q24;q11) translocations associated with certain acute T-cell leukemias based on an alteration in the size of the BamHI fragment detected.
Vector
Construct size (kb): 7.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 5.0
Tissue: placenta
Gene product: T cell receptor, delta (V,D,J,C) [TCRD]
Insert Size (kb)
5.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.6; EcoRI--5.0, 2.8.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--1.1, 1.8, 3.0; HindIII--3.5, 4.0.
The MOLT-4 cell line (ATCC CRL-1582) is deleted for this fragment.
Detects the following human genomic germline restriction fragments (kb): BamHI--16.5; HindIII--5.8, 3.2.
Has been used to detect t(10;14)(q24;q11) translocations associated with certain acute T-cell leukemias based on an alteration in the size of the BamHI fragment detected.
Corresponds to the EcoRI fragment between Jdelta1 and Jdelta2.
References

Kagan J, et al. Clustering of breakpoints on chromosome 10 in acute T-cell leukemias with the t(10;14) chromosome translocation. Proc. Natl. Acad. Sci. USA 86: 4161-4165, 1989. PubMed: 2542965

Croce CM. Molecular probing for human T-cell leukemia and lymphoma. US Patent 5,198,338 dated Mar 30 1993

Croce CM. TCL-5 gene rearrangement involved in T-cell leukemia and melanoma. US Patent 5,242,795 dated Sep 7 1993

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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