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NCI-H1522 [H1522]
NCI-H1522 [H1522]
規(guī)格:
價(jià)格:
編號(hào):B209338
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H1522 [H1522]
商品貨號(hào)
B209338
Organism
Homo sapiens, human
Tissue
lung
培養(yǎng)基
Product Format
flask
Morphology
rounded
Culture Properties
mixed adherent-suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E,carcinoma; small cell lung cancer
Age
53 years
Gender
male
Ethnicity
Caucasian
Applications
The line was established in October 1986.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established in October 1986.
Clinical Data
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
male
Caucasian
53 years
Comments
The line was established in October 1986.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
60 pack years.
Complete Growth Medium
HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: XY
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9, 11, 12
THO1: 7, 9.3
TPOX: 8, 11
vWA: 17, 20
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1986
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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