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LL288
LL288
規格:
價格:
編號:B209348
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
LL288
商品貨號
B209348
Designations
LL288
GenBank Number

U30610

培養基
Species
Homo sapiens, human
Depositors
LL Lanier
Vector
Construct size (kb): 4.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJFE14
Intact vector size: 3.500
Type of vector: plasmid
Vector end: BstXI
Vector end: NotI
Cloning sites: BstXI NotI SacII SfiI EcoRI
Polylinker sites: BstXI NotI SacII SfiI EcoRI
Construction: pcDL-SRalpha296
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
replicon: SV40
promoter for expression: SV40 early region
enhancer: HTLV-1 R-U5 segment of LTR
MCS: BstXI...EcoRI
terminator: SV40 late region polyadenylation signal
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD94
Gene name: CD94 antigen (NK/T-cell C-type lectin receptor)
ORF's seq. position: GDB probe: GDB:696221 ()
Contains complete coding sequence?: Y
Chromosome: 12; Localization: 12 p13
Tissue: polyclonal NK cell line
Type of DNA: cDNA
Insert 5' end: Modification: BstXI linker
Insert 3' end: Modification: NotI linker
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: U30610
Nucleotides 1-834 of the insert correspond to
nucleotides 1-834 of U30610.
Insert lengths(kb): 0.800000011920929
Tissue: polyclonal NK cell line
Gene product: CD94 antigen (NK/T-cell C-type lectin receptor) [CD94]
Insert Size (kb)
0.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BstXI--4.2; BstXI/NotI--3.4, 0.84; NotI--4.2.
The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.28; SacI--0.41.
References

Chang C, et al. Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily. Eur. J. Immunol. 25: 2433-2437, 1995. PubMed: 7589107

Lanier LL, et al. Human NKR-P1A. A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes. J. Immunol. 153: 2417-2428, 1994. PubMed: 8077657

Takebe Y, et al. SRalpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol. 8: 466-472, 1988. PubMed: 2827008

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