Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,cmlR
Construct size (kb): 4.842000007629395
Features: marker(s): ampR other: omega element promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pMB1 MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: cmlR (cat)
Applications
contains easily purifiable cassette(s) for construction omega-cat
Comments
Restriction digests of the clone give the following sizes (kb): MluI--3.1, 1.8; EcoRI--2.9, 1.9; BamHI--2.9, 1.8.
Contains an omega-Cm element acetyltransferase (cmlR) and multiple cloning sites duplicated in tandem orientation.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
The EcoRI site was removed.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
