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T-VCAM-1 [clone L-59]
T-VCAM-1 [clone L-59]
規(guī)格:
價(jià)格:
編號(hào):B209554
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
T-VCAM-1 [clone L-59]
商品貨號(hào)
B209554
Designations
T-VCAM-1 [clone L-59]
GenBank Number

U12884

培養(yǎng)基
Species
Mus musculus, mouse
Depositors
CM Ballantyne
Applications
contains sequence vascular cell adhesion molecule-1 vascular cell adhesion molecule 1
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.399999976158142
Tissue: liver, LPS-treated mouse
Gene product: vascular cell adhesion molecule-1(vascular cell adhesion molecule 1) [Vcam1]
Target Gene: vascular cell adhesion molecule-1
Insert Size (kb)
1.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): NdeI--4.3; SphI--4.4; SacI/XhoI--2.9, 1.4.
Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.35; BglII--0.08; HindIII--0.07; NcoI--0.12, 0.24; NdeI--1.00; PvuI--0.10; SphI--1.20; XbaI--0.52.
Encodes a truncated form of Vcam1 including Ig domains 1-3 and having a different carboxy terminus.
The 5' end of the gene was generated by PCR and was ligated to the 3' portion of the clone N10 at the BamHI site. The resulting full length cDNA was confirmed by sequencing.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Kumar AG, et al. Murine VCAM-1. Molecular cloning, mapping, and analysis of a truncated form. J. Immunol. 153: 4088-4098, 1994. PubMed: 7523515

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