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pUCD2 plasmid in E. coli

規(guī)格:

價(jià)格:

編號(hào):B209795

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pUCD2 plasmid in E. coli

商品貨號(hào)

B209795

Designations

pUCD2 plasmid in E. coli

Depositors

CI Kado

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 13.0

Vector: pUCD2 (plasmid)

Promoters: Promoter none

Construction: pBR322, pSal151

Marker(s):ampR,kanR,spcR,tetR

Construct size (kb): 13.0

Features: marker(s): ampR, kanR, spcR, tetR
promoter: none
replicon: pMB1, pSa151
terminator: none
enhancer: none

Applications

shuttle vector

Comments

A broad host range amplifiable cloning vector.

This is a broad host range amplifiable cloning vector for use in complementation studies of cloned inserts in Agrobacterium tumefaciens.

It was constructed by fusing pBR322 with pSAl51 plasmid and was shown to have lost 2.5 kb on each side of the BglII site giving it a positive vir region complementation in Agrobacterium tumafaciens.

Restriction digests of the clone give the following sizes (kb): PstI--13; EcoRV--13; KpnI--13; SalI--13.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Close TJ, et al. Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58. Plasmid 12: 111-118, 1984. PubMed: 6095350

Shipped

freeze-dried

Shipping Information

Shipped: ?Freeze dried E. coli containing the cosmid

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