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YRp7 [pRB16]
YRp7 [pRB16]
規格:
價格:
編號:B209807
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
YRp7 [pRB16]
商品貨號
B209807
Designations
YRp7 [pRB16]
Depositors
D Botstein
培養基
Biosafety Level
1
Vector Information
Size (kb): 5.817
Vector: YRp7 (plasmid)
Promoters: none
Construction: pBR322, lambdagt-Sc4101
Marker(s):TRP1,ampR,tetR
Construct size (kb): 5.817
Features: marker(s): TRP1
marker(s): ampR
marker(s): tetR
promoter: none
replicon: ARS1
replicon: ROP
replicon: pMB1
terminator: none
enhancer: none
Applications
YR-type (replicating) shuttle vector
shuttle vector
Comments
A general purpose replicating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
YRp7 was constructed by inserting a 1.4 kb EcoRI fragment containing TRP1 and ARS1 from lambdagt-Sc4101 into pBR322. The ARS1 fragment is in a 0.85 kb EcoRI-HindIII subfragment.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped
freeze-dried
Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

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