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lambdaPEC-3 [lambdahPEC-3]
lambdaPEC-3 [lambdahPEC-3]
規格:
價格:
編號:B210070
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
lambdaPEC-3 [lambdahPEC-3]
商品貨號
B210070
Designations
lambdaPEC-3 [lambdahPEC-3]
GenBank Number

L05144

培養基
Species
Homo sapiens, human
Depositors
GI Bell
Vector
Construct size (kb): 46.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt10
Intact vector size: 43.340
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.6 kb
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: lambda
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PCK1
Gene name: phosphoenolpyruvate carboxykinase 1
Contains complete coding sequence?: Y
Chromosome: 20; Localization: 20 q13.2-q13.31
Tissue: kidney
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.7
Cross references: DNA Seq. Acc.: L05144
Insert lengths(kb): 2.700000047683716
Tissue: kidney
Gene product: phosphoenolpyruvate carboxykinase 1, soluble(phosphoenolpyruvate carboxykinase (GTP)) [PCK1]
Insert Size (kb)
2.700
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--16.0, 9.0, 6.8 (doublet), 5.6, 1.5; EcoRI--32.0, 11.0, 2.0, 0.8; HindIII--23.0, 9.3 (doublet), 4.5; NotI--34.0, 12.0;
PstI--11.0, 5.6 (doublet), 2.8 (doublet), 2.5 (doublet), 2.1, 1.8, 1.5, 1.3 (doublet, 1.2, 1.0 (doublet), 0.85; PvuII--11.0 (doublet), 4.5 (doublet), 4.1, 3.8, 1.8, 1.4; SacI--33.0, 13.0.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.15, 1.52; PvuII--0.13, 1.47; NotI--0.97; SacI--1.3; EcoRI--1.87.
Contains the complete coding sequence (nt 122-1990).
The 3' untranslated region contains a simple tandem repeat polymorphism that is highly polymorphic.
References

Stoffel M, et al. cDNA sequence and localization of polymorphic human cytosolic phosphoenolpyruvate carboxykinase gene (PCK1) to chromosome 20, band q13.31: PCK1 is not tightly linked to maturity-onset diabetes of the young. Hum. Mol. Genet. 1: 1-4, 1993. PubMed: 8490617

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