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pDMR
pDMR
規格:
價格:
編號:B210095
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pDMR
商品貨號
B210095
Designations
pDMR
GenBank Number

M23412

培養基
Species
Drosophila melanogaster, fruit fly
Depositors
JC Venter
Vector
Construct size (kb): 7.800000190734863
Insert
DNA: cDNA
Insert lengths(kb): 4.800000190734863
Gene product: cholinergic receptor, muscarinic [CHRM*]
Insert Size (kb)
4.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.8, 3.5, 0.6; BamHI--6.0, 2.4, 0.3; HindIII--6.3, 1.6; SmaI--7.8; EcoRI/SmaI--7.8; EcoRI--7.8; EcoRI/PstI--3.4, 3.0, 0.6 (doublet), 0.3.
The insert contains the following restriction sites (nt position according to the published sequence): PstI--434, 700, 1210, 1711; BamHI--2596; HindIII--3316.
A full-length cDNA clone which includes approximately 0.3 kb 5' and 2.1 kb 3' untranslated sequence.
Constructed by blunt-ending an EcoRI fragment before inserting it into the vector.
References

Onai T, et al. Chromosome localization of a Drosophila muscarinic cholinergic receptor. FEBS Lett. 255: 219-225, 1989. PubMed: 2507354

J C Venter, personal communication

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