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pG1-3l3
pG1-3l3
規(guī)格:
價(jià)格:
編號(hào):B210142
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pG1-3l3
商品貨號(hào)
B210142
Designations
pG1-3l3
Species
Mus musculus, mouse
培養(yǎng)基
Depositors
MH Meisler
Vector
Construct size (kb): 3.5
Insert
DNA: genomic
Insert lengths(kb): 0.5109999775886536
Gene product: amylase-1, salivary [Amy1]
Insert Size (kb)
0.511
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.5; EcoRI--3.5; HindIII--3.5; PvuII--3.5; PvuI/PvuII--2.0, 1.5.
The 5' boundary of the insert is the HindIII site at nt -374 relative to the liver-specific non-coding exon; the 3' boundary is the EcoRI site at nt +131 within that exon.
The insert includes 2 regions showing homology to the glucocorticoid consensus sequence.
Transcription from the T7 promoter gives an antisense riboprobe. The fragment was subcloned from the cosmid cSamD4.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Darlington GJ, et al. Simultaneous expression of salivary and pancreatic amylase genes in cultured mouse hepatoma cells. Mol. Cell. Biol. 6: 969-975, 1986. PubMed: 2431276

Samuelson LC, et al. Glucocorticoid and developmental regulation of amylase mRNAs in mouse liver cells. Mol. Cell. Biol. 8: 3857-3863, 1988. PubMed: 2464743

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