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pTW11 [CMCC 2210]
pTW11 [CMCC 2210]
規(guī)格:
價(jià)格:
編號(hào):B210184
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pTW11 [CMCC 2210]
商品貨號(hào)
B210184
Designations
pTW11 [CMCC 2210]
GenBank Number

M73789

培養(yǎng)基
Species
Streptomyces rubiginosus (Preobrazhenskaya et al.) Pridham et al.
Depositors
Cetus Corp., K Van Note, Cetus Corp.
Applications
produces protein D-xylose isomerase
Vector
Construct size (kb): 5.659999847412109
Insert
DNA: genomic
Insert lengths(kb): 1.200000047683716
Gene product: D-xylose isomerase [xylA]
Target Gene: D-xylose isomerase
Insert Size (kb)
1.200
Media
ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 1.2; SalI--4.8, 0.7; XbaI--uncut.
To induce expression of the insert, grow in LB with limiting concentrations of tryptophan.
A genomic NruI/SmaI fragment was modified and cloned into pTrp3. Contrary to the description published in J. Bacteriology 173, 6849-6858, 1991, this construct appears to have had HindIII sites engineered up and downstream of the coding region.
References

Drummond RJ, et al. Procaryotic xylose isomerase muteins. US Patent 5,041,378 dated Aug 20 1991

Wong HC, et al. Genetic organization and regulation of the xylose degradationgenes in Streptomyces rubiginosus. J. Bacteriol. 173: 6849-6858, 1991. PubMed: 1657868

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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