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pAQP4

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編號:B210344

品牌:Mingzhoubio

標準菌株

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DNA

RNA

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產品名稱

pAQP4

商品貨號

B210344

Designations

pAQP4

GenBank Number

U14007

培養基

Species

Rattus norvegicus, rat

Depositors

P Agre, GM Preston

Vector

Construct size (kb): 4.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: AQP4
Gene name: aquaporin 4
Contains complete coding sequence?: N
Tissue: brain
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.6
Cross references: DNA Seq. Acc.: U14007
Nucleotides 1-1588 of the insert correspond to
nucleotides 7-1595 of U14007.

Insert lengths(kb): 1.600000023841858

Tissue: brain

Gene product: aquaporin 4 [AQP4]

Insert Size (kb)

1.600

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.6; HindIII--4.2, 0.3; SmaI--3.7, 0.85; KpnI--4.6; SstI--3.8, 0.8.

Encodes a mercury-resistant water channel expressed primarily in rat adult brain.

References

Jung JS, et al. Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance. Proc. Natl. Acad. Sci. USA 91: 13052-13056, 1994. PubMed: 7528931

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