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22D1
22D1
規格:
價格:
編號:B210349
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
22D1
商品貨號
B210349
Organism
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type
hybridoma:lymphoblast B lymphocyte; somatic cell hybri
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is useful for FACS and immunocytochemistry assays.
The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays.
Comments
Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) to 4 X 10(4) viable cells/ml.
Interval: Subculture when cells reach a concentration of 8 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
hamster, Armenian IgG
Population Doubling Time
12 hours
Name of Depositor
CG Park, RM Steinman
Year of Origin
May, 2003
References

Kang YS, et al. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc. Natl. Acad. Sci. USA 101: 215-220, 2004. PubMed: 14694198

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